Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 18
Release date: Oct 20 2013
Last update date: Aug 9 2018
Access: Public
Taxon: Homo sapiens
Dataset link Global profiling of human hair follicle scalp dermal papilla cells using Affymetrix microarrays

Experimental Protocol


Affymetrix microarrays were used to to perform profiling of human dermal papilla cells, both as intact tissues (freshly isolated from scalp), and at several stages in subsequent two dimensional culture; cell explant outgrowths (p0), cells at passage 1 (p1), passage 3 (p3) and passage 5 (p5). RNA was isolated from cultured cells 72 hours after feeding. Cells at passage 3 were also grown in hanging drops to form dermal spheroids, that were used for RNA collection 48 hours after establishment. All experiments were performed using tissue from three biological replicates (#D5, D6, D7),

Repositories


GEO

GSE44765

ArrayExpress

E-GEOD-44765

BioProject

PRJNA191608

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Contact


Claire Higgins
Claire A Higgins