Computational protocol: Identification of reference genes for circulating microRNA analysis in colorectal cancer

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Protocol publication

[…] Statistical analysis was carried out using the GraphPad Prism 5 using two-tailed Student’s test. Data were present as means ± SE (standard error). P value of less than 0.05 was considered statistically significant. Mean values were obtained from SPSS software, version19.0 (SPSS Inc., Chicago, IL, USA). The stability of the candidate reference genes was evaluated by the computer program BestKeeper, geNorm and NormFinder. In the BestKeeper software, data observations are in the form of raw threshold cycles, while fold change were used as input data in both geNorm and NormFinder. […]

Pipeline specifications

Software tools BestKeeper, NormFinder
Application qPCR
Organisms Homo sapiens
Diseases Breast Neoplasms, Carcinoma, Non-Small-Cell Lung, Neoplasms, Colorectal Neoplasms