Dataset features

Specifications


Application: WES analysis
Number of samples: 80
Release date: Jan 30 2017
Last update date: May 23 2017
Access: Public
Diseases: Liver Neoplasms, Neoplasms, Hepatoblastoma
Dataset link SNP profiles of 50 HB tumors

Experimental Protocol


88 clinically-annotated frozen pre-clinical HB specimens with corresponding surgical pathology reports were included in the study (Table S1) following histological review. Specimens and clinical annotation were obtained from the COG Tumor Biospecimen Bank in Columbus, Ohio, the Pediatric Oncology Group Hepatoblastoma Biology Study P9346, and Texas-based institutions, under IRB-approved protocols. Whole exome sequencing (WES) was performed on 35 tumors for which matching normal DNA – either a blood sample or tumor-adjacent normal liver – was available (Table S2). Targeted sequencing of the CTNNB1 and NFE2L2 genes and the TERT promoter was completed on all 88 tumors. DNA from 47 HBs, including all 35 samples subjected to WES, was also analyzed by SNP array. RNA and miRNA expression profiling were performed on 50 and 57 tumors, respectively.

Repositories


GEO

GSE75284

BioProject

PRJNA303089

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Contact


Toni-Ann Mistretta