Dataset features

Specifications


Application: RNA-seq analysis
Number of samples: 18
Release date: Nov 7 2018
Last update date: Nov 30 2018
Access: Public
Diseases: Craniofacial Dysostosis, Osteoporosis
Genes: M8*, FGFR2, ENPP1, ANK1
Mutations: p|SUB|S|252|W
Taxon: Mus musculus
Dataset link Mandibular dysmorphogenesis due to abnormal osteogenic activity in FGFR2-related craniosynostosis mouse models

Experimental Protocol


Quantitative high-resolution micro-computed tomography (µCT) images of the mandible were acquired for 182 newborn (P0) mice to investigate the effects of three FGFR2 mutations associated with Apert and Crouzon syndromes. Samples for histological and transcriptome analysis consisted of 66 embryos of Fgfr2+/S252W model. Laser Capture Microdissection was used on embryos at E16.5 (3 Fgfr2S252W embryos and 3 unaffected littermates, all were female) to isolate tissues in Meckels cartilage, mandibular bone and mandibular condylar cartilage, respectively. Libraries were prepared with NuGEN Ovation RNA-Seq System v2 (amplification) and Illumina Nextera XT Library Prep kit. Fragments were sequenced using paired end reads (2×100 bp) on the Illumina HiSeq platform.

Repositories


GEO

GSE121780

ENA

SRP166854

BioProject

PRJNA498423

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Contact


Harm Bakel