|Number of samples:||18|
|Release date:||Nov 7 2018|
|Last update date:||Nov 30 2018|
|Diseases:||Craniofacial Dysostosis, Osteoporosis|
|Genes:||M8*, FGFR2, ENPP1, ANK1|
|Dataset link||Mandibular dysmorphogenesis due to abnormal osteogenic activity in FGFR2-related craniosynostosis mouse models|
Quantitative high-resolution micro-computed tomography (µCT) images of the mandible were acquired for 182 newborn (P0) mice to investigate the effects of three FGFR2 mutations associated with Apert and Crouzon syndromes. Samples for histological and transcriptome analysis consisted of 66 embryos of Fgfr2+/S252W model. Laser Capture Microdissection was used on embryos at E16.5 (3 Fgfr2S252W embryos and 3 unaffected littermates, all were female) to isolate tissues in Meckels cartilage, mandibular bone and mandibular condylar cartilage, respectively. Libraries were prepared with NuGEN Ovation RNA-Seq System v2 (amplification) and Illumina Nextera XT Library Prep kit. Fragments were sequenced using paired end reads (2×100 bp) on the Illumina HiSeq platform.