Computational protocol: Molecular mutation profile of pfcrt in Plasmodium falciparum isolates imported from Africa in Henan province

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Protocol publication

[…] Sequence alignments and analysis were carried out using BioEdit software. Amino acid sequences were compared with 3D7 strain, which was obtained from the Chinese Centre for Disease Control and Prevention. The sequences of the amplicons were aligned with 3D7 strain published data from the NCBI database by BLAST analysis. All the data were proceed using Excel to build database and the SPSS 17.0 software was used for analysis. The Person’s Chi square test was used to determine significance of results. A P value <0.05 was considered statistically significant. […]

Pipeline specifications

Software tools BioEdit, SPSS
Application Miscellaneous
Organisms Plasmodium falciparum, Homo sapiens
Diseases Infection, Malaria, Malaria, Falciparum
Chemicals Chloroquine, Nucleotides