Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 6
Release date: Mar 23 2012
Last update date: Mar 6 2018
Access: Public
Diseases: Fatty Liver, Chronobiology Disorders
Chemicals: Glucose
Dataset link Expression data from hepatocyte specific Klf6 knockout mice vs. control on high fat diet

Experimental Protocol


Three mice with a hepatocyte-specific knockdown of KLF6 (DeltaHepKlf6) on high fat diet and 3 littermate controls on the same diet were sacrificed after 8 weeks of diet. Liver tissue was preserved in RNAlater® (Ambion, Austin, TX). RNA was isolated from liver tissue and homogenized in TRIzol® reagent (Invitrogen, Carlsbad, CA). In order to identify potential KLF6 targets that contributed to changes in glucose- and lipid-metabolism, we performed an Affymetrix Exon1 S.T. Genearray® (Affymetrix, Santa Clara, CA).

Repositories


GEO

GSE36724

ArrayExpress

E-GEOD-36724

BioProject

PRJNA153799

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Contact


Weijia Zhang