Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 21
Release date: Dec 1 2012
Last update date: Nov 9 2017
Access: Public
Diseases: Neoplasms, Germ Cell and Embryonal
Chemicals: Calcium, Potassium, Puromycin, Sodium
Dataset link Transcriptomic profiling of murine ES and iPS cells, embryoid bodies, and ES and IPS cell-derived cardiomyocytes

Experimental Protocol


Seven different experimental groups were included into analysis: undifferentiated murine ES cells (1) and undifferentiated murine iPS cells (2), murine ES cell-derived embyroid bodies (3) and murine iPS cell-derived embryoid bodies at day 16 of differentiation (4), murine ES cell-derived cardiomyocytes (5) and murine iPS cell-derived cardiomyocytes (6) at day 16 of differentiation (they were generated by puromycin selection for 7 days prior to RNA isolation). Adult mouse tail tip fibroblasts (7) were used as a control for iPS cells. Total RNA samples were prepared from three independent biological replicates in groups 1-6. In group 7, single RNA probes were analyzed as three technical replicates.

Repositories


GEO

GSE18514

ArrayExpress

E-GEOD-18514

BioProject

PRJNA118277

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Contact


Joachim Schultze