|Application:||Gene expression microarray analysis|
|Number of samples:||24|
|Release date:||Aug 23 2011|
|Last update date:||May 11 2017|
|Diseases:||Metabolic Diseases, Neoplasms, Adipose Tissue|
|Dataset link||Gene expression from mouse white, brown, and perivascular adipose tissue|
8-week-old male C57BL6/J mice were fed a normal (ND) or high fat diet (HFD) (Research Diets 12451, 45 kcal% fat) for 13 weeks. Mice were then euthanized and four different adipose depots were harvested for RNA analysis: perivascular fat from the lesser curvature of the aortic arch (PVAT), interscapular brown adipose (BAT), inguinal adipose tissue (SAT), and epididymal adipose tissue (VAT). 250 ng total RNA pooled from two mice was used for cDNA synthesis; 3 biological replicates per tissue and diet were performed for a total of 24 hybridizations.
Michael P Czech