Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 24
Release date: Aug 23 2011
Last update date: May 11 2017
Access: Public
Diseases: Metabolic Diseases, Neoplasms, Adipose Tissue
Dataset link Gene expression from mouse white, brown, and perivascular adipose tissue

Experimental Protocol


8-week-old male C57BL6/J mice were fed a normal (ND) or high fat diet (HFD) (Research Diets 12451, 45 kcal% fat) for 13 weeks. Mice were then euthanized and four different adipose depots were harvested for RNA analysis: perivascular fat from the lesser curvature of the aortic arch (PVAT), interscapular brown adipose (BAT), inguinal adipose tissue (SAT), and epididymal adipose tissue (VAT). 250 ng total RNA pooled from two mice was used for cDNA synthesis; 3 biological replicates per tissue and diet were performed for a total of 24 hybridizations.

Repositories


GEO

GSE28440

ArrayExpress

E-GEOD-28440

BioProject

PRJNA139249

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Contact


Timothy Fitzgibbons
Michael P Czech