Computational protocol: The occurrence of spring forms in tetraploid Timopheevi wheat is associated with variation in the first intron of the VRN-A1 gene

Similar protocols

Protocol publication

[…] Multiple sequence alignments and the subsequent phylogenetic analysis were carried out using the ClustalW program and MEGA4 software [, ]. A phylogenetic tree was constructed using the Neighbor-Joining algorithm and 500 bootstrap replicates. Divergence time was assessed by MEGA4 using calibration against a previously established date of formation of the allotetraploid wheat of about 0.5-0.6 Million Years Ago (MYA) [, ]. […]

Pipeline specifications

Software tools Clustal W, MEGA
Application Phylogenetics
Organisms Triticum timopheevii, Triticum aestivum