Dataset features

Specifications


Application: RNA-seq analysis
Number of samples: 3
Release date: Sep 19 2013
Last update date: Jul 26 2018
Access: Public
Diseases: Leukemia, Leukemia, Myeloid, Acute, Hematologic Neoplasms, Anemia, Diamond-Blackfan
Dataset link Targeting oncogene expression to endothelial cells induces proliferation of the myelo-erythroid lineage by repressing the notch pathway

Experimental Protocol


Methods: mRNA profiles of transgenic zebrafish overexpressing the oncogene HRAS in endothelial cells (Tg(fli1ep:GAL4FF)ubs3; Tg(UAS:eGFP-HRASV12)io006); or expressing activate Notch in endothelial cells (Tg(fli1ep:GAL4FF)ubs3; tg(UAS:NICD)kca3) were generated by deep sequencing using Illumina HiSeq 2000. The sequence reads that passed quality filters were analyzed using the CLC bio Assembly Cell software (version 3.2) and the Ensembl (release 63) predicted cDNAs for the Zv9 genome assembly. qRT–PCR validation was performed using TaqMan and SYBR Green assays.

Repositories


GEO

GSE41988

ArrayExpress

E-GEOD-41988

ENA

SRP017013

BioProject

PRJNA178664

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Contact


marina mione