|Application:||Gene expression microarray analysis|
|Number of samples:||2|
|Release date:||Jul 31 2009|
|Last update date:||Aug 23 2018|
|Genes:||ZBTB18, Kv5.1, ID1, ID2, ID3, CKM, MYH14|
|Dataset link||RNA profiling of skeletal muscle in RP58 knockout mice|
Skeletal muscles of WT and RP58 KO mice (E18.5 diaphragm) were dissected under a microscope and RNA was extracted using ISOGEN (Nippongene). After EtOH precipitation, RNA was dissolved into DEPC-DW and its concentration was determined. Microarray analysis - cRNA was synthesized. 10 ug of cRNA were hybridized to Affymetrix mouse 430A 2.0 arrays. Signal intensities were calculated using the RMA method. MAPPFinder (www.genmapp.org) was used to integrate expression data with known pathways.