Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 2
Release date: Jul 31 2009
Last update date: Aug 23 2018
Access: Public
Genes: ZBTB18, Kv5.1, ID1, ID2, ID3, CKM, MYH14
Dataset link RNA profiling of skeletal muscle in RP58 knockout mice

Experimental Protocol


Skeletal muscles of WT and RP58 KO mice (E18.5 diaphragm) were dissected under a microscope and RNA was extracted using ISOGEN (Nippongene). After EtOH precipitation, RNA was dissolved into DEPC-DW and its concentration was determined. Microarray analysis - cRNA was synthesized. 10 ug of cRNA were hybridized to Affymetrix mouse 430A 2.0 arrays. Signal intensities were calculated using the RMA method. MAPPFinder (www.genmapp.org) was used to integrate expression data with known pathways.

Repositories


GEO

GSE16595

ArrayExpress

E-GEOD-16595

BioProject

PRJNA116161

Download


Contact


Hiroshi Asahara