Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 2
Release date: Mar 1 2014
Last update date: Aug 13 2018
Access: Public
Dataset link Identification of hypoxia target genes in human CB CD34+ cells

Experimental Protocol


CB CD34+ cells were isolated by Miltenyi miniMACS column. Cells were prestimulated in HPGM with 100 ng/ml KITL, FLT3L and TPO for 3 days after which cells were placed either at normoxia or hypoxia (1% O2 for an additional 24 hrs). 5 independent CB CD34+ batches were used and isolated RNA was combined and used for Illumina beadhchip arrays HT12 v4

Repositories


GEO

GSE54661

ArrayExpress

E-GEOD-54661

BioProject

PRJNA237311

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Contact


JJ Schuringa