Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 18
Release date: Mar 1 2010
Last update date: Oct 29 2018
Access: Public
Diseases: Neoplasms, Lymphoma, B-Cell, Marginal Zone, Renal Insufficiency
Dataset link Examining smoking-induced differential gene expression changes in buccal mucosa

Experimental Protocol


Samples were collected from eight subjects, four smokers (Sm)and four nonsmokers (NS). Each cheek was sampled creating an a and b sample for each subject which is reflected in the array name. All samples were isolated separately for total RNA. Each was hybridzed to microarrays to examine for differential gene expression between smokers and nonsmokers. There are 14 total samples in the main dataset (Set 2). One cheek sample failed in microarray analysis for two individuals, 08BCNS23 a and 08BCSm27 a, and so are not included here. A sample set (Set 1) was created which contains the four samples shown in this file. They represent repeated sampling of both cheeks for two individuals to test for reproducibility. There is a separate RMA file and metadata file (this file) for these data. These included samples 08BC11Sm a and b, a smoker, and 08BC12NSa and b a nonsmoker.

Repositories


GEO

GSE16149

ArrayExpress

E-GEOD-16149

BioProject

PRJNA115079

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Contact


Dennis Burian
Doris Kupfer

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