Computational protocol: Six3 demarcates the anterior-most developing brain region in bilaterian animals

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Protocol publication

[…] All primers, PCR programs and template DNA source are given in Additional file . Tc-six3 gene was identified by in silico analysis of the Tribolium genome and amplified from a mixed stages (0 to 24h) cDNA library. Full length Pdu-six3 was isolated by screening a 48 h λ-ZAP phage library (provided by C. Heimann, Mainz). Pdu-tryptophane-2,3-dioxygenase gene was identified during a sequencing screen of a 48 h Platynereis EST library. Gene orthology was confirmed by using NCBI Protein BLAST, MUSCLE [] multiple sequence alignments and CLUSTALX v.2 neighbour-joining phylogenetic analysis [] for complete proteins. […]

Pipeline specifications

Software tools MUSCLE, Clustal W
Databases NCBI Protein
Applications Phylogenetics, Nucleotide sequence alignment
Organisms Platynereis dumerilii, Drosophila melanogaster