|Application:||Gene expression microarray analysis|
|Number of samples:||12|
|Release date:||Aug 22 2010|
|Last update date:||Oct 29 2018|
|Diseases:||Fibromyalgia, Scleroderma, Systemic|
|Dataset link||Expression data from untreated and rottlerin-treated normal and systemic sclerosis-derived human fibroblasts|
Early passage normal and SSc human dermal fibroblasts were incubated with rottlerin (50 µg/mL) for 24 hours, followed by RNA extraction of treated and untreated samples and hybridization on Affymetrix microarrays. Early passage fibroblasts derived from normal and SSc dermal biopsies were utilized in this study to ensure that phenotypes remained as close as possible to original biopsy tissue. SSc patient sample biopsies were isolated from leading edge of forearm lesion; normal biopsies were obtained from forearm dermis.