Computational protocol: The Glycogen Synthase Kinase 3α and β Isoforms Differentially Regulates Interleukin-12p40 Expression in Endothelial Cells Stimulated with Peptidoglycan from Staphylococcus aureus

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Protocol publication

[…] The relative abundance of phosphorylated proteins was quantitated by densitometric analysis with the Image Processing and Analysis in Java Program ImageJ (http://rsbweb.nih.gov/ij). To calculate the densitometric values, the intensity of the phosphorylated band was divided by the intensity of the non-phosphorylated ones. These intensity values were referred to a value of 1.0 that was arbitrarily assigned to the untreated control. The statistical significance of triplicate blots, IL-12p40 mRNA levels and IL-12p40 protein levels were evaluated with the Tukey multiple-comparison test and One-Way analysis of variance (ANOVA) by using the SIGMASTAT program version 3.0 (SPSS Inc., Chicago, IL, USA). P values <0.05 or <0.01 or <0.001 were considered statistically significant. […]

Pipeline specifications

Software tools ImageJ, SPSS
Applications Miscellaneous, Microscopic phenotype analysis
Organisms Staphylococcus aureus, Bos taurus, Epipremnum aureum
Diseases Cardiovascular Diseases, Diabetes Mellitus, Neoplasms
Chemicals Lithium Chloride