|Application:||Gene expression microarray analysis|
|Number of samples:||48|
|Release date:||Feb 27 2018|
|Last update date:||Oct 29 2018|
|Diseases:||Adenoma, Duodenal Neoplasms, Duodenitis, Intestinal Diseases, Neoplasms, Adenomatous Polyposis Coli|
|Dataset link||Gene Expression Changes in the Duodenal Adenoma-Carcinoma Sequence in Familial Adenomatous Polyposis (FAP)|
Gene expression analysis was performed on duodenal samples of 12 FAP patients diagnosed with duodenal cancer (cases) between 1988-2014 and 12 FAP patients without duodenal cancer (controls). RNA was extracted from archived normal, adenoma and cancer tissue from cases and from archived adenoma tissue from controls. Gene expression profiling and analysis was performed using the Affymetrix Human Transcriptome Array 2.0. Three pairwise comparisons were conducted to describe the adenoma-carcinoma sequence: normal-adenoma (No-Ad), adenoma-cancer (Ad-Ca) and normal-cancer (No-Ca). To determine genes differentially expressed in FAP patients with duodenal cancer, adenoma from cases was compared with adenoma from controls (Ad-Ad). In total, 48 samples were analyzed, 36 from cases (normal, adenoma, cancer) and 12 from controls (adenoma).