Computational protocol: Optimization of Culture Conditions for Production of the Anti-Leukemic Glutaminase Free L-Asparaginase by Newly Isolated Streptomyces olivaceus NEAE-119 Using Response Surface Methodology

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Protocol publication

[…] The complete 16S rRNA gene sequence (1509 bp) of strain NEAE-119 was aligned with the corresponding 16S rRNA sequences of the type strains of representative members of the genus Streptomyces retrieved from the GenBank, EMBL, DDBJ, and PDB databases by using BLAST program ( [] and the software package MEGA4 version 2.1 [] was used for multiple alignment and phylogenetic analysis. The phylogenetic tree was constructed via the bootstrap test of neighbor-joining algorithm [] based on the 16S rRNA gene sequences of strain NEAE-119 and related organisms. [...] The experimental data obtained was subjected to multiple linear regressions using Microsoft Excel 2007. The P values were used as a tool to check the significance of the interaction effects, which in turn may indicate the patterns of the interactions among the variables []. The statistical software package, STATISTICA software (Version 8.0, StatSoft Inc., Tulsa, USA), was used to plot the three-dimensional surface plots. […]

Pipeline specifications

Software tools MEGA, Statistica
Databases DDBJ
Applications Miscellaneous, Phylogenetics
Organisms Saccharomyces cerevisiae
Diseases Precursor Cell Lymphoblastic Leukemia-Lymphoma
Chemicals Glucose, Sodium Chloride