Dataset features

Specifications


Application: SNP array data analysis
Number of samples: 12
Release date: Dec 19 2007
Last update date: Mar 17 2012
Access: Public
Diseases: Enteritis, Gastroenteritis, Miller Fisher Syndrome, Angina, Stable
Computational protocol: BLASTN, Clustal W, MEGA, PubMLST
Dataset link Comparative Genomic Analysis of Clinical Strains of Campylobacter jejuni from South Africa

Experimental Protocol


The genomic diversity of 33 South African, 2 Mexican and 1 Canadian Campylobacter jejuni strains from humans were examined by microarray-based comparative genomic indexing (CGI) analysis. The CGI analysis allowed the assessment of CDS content for each C. jejuni strain relative to the C. jejuni DNA microarray, which comprises ORFs from strains NCTC 11168, RM1221. ORFs were spotted in duplicate. Genomic DNA from strains NCTC 11168/RM1221 were used as a reference DNA and competitively hybridized with genomic DNA from each of the other C. jejuni strains. Two or three replicates for each strain were performed. Data normalization was performed as in Parker et al. J Clin Microbiol 2006, 44(11):4125-4135.

Repositories


GEO

GSE9862

ArrayExpress

E-GEOD-9862

BioProject

PRJNA103847

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Contact


Craig Parker
Craig T Parker

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