Dataset features

Specifications


Application: RNA-seq analysis
Number of samples: 4
Release date: Aug 6 2014
Last update date: Jul 26 2018
Access: Public
Diseases: Deficiency Diseases, Substance Abuse, Intravenous
Dataset link Phagocytosis of mycobacteria by zebrafish macrophages is dependent on the scavenger receptor Marco, a key control factor of pro-inflammatory signalling

Experimental Protocol


Embryos were injected at the one cell stage with a morpholino targeting marco, or with the standard control morpholino from GeneTools for comparison. Subsequently, at 24 hours post fertilization (hpf) the morphants and their controls were manually dechorionated at 24 hpf and at 28 hpf they were infected by injecting 200 colony forming units of M. marinum Mma20 into the caudal vein, or mock-injected with PBS/2%PVP. After injections embryos were transferred into fresh egg water containing 0.003% 1-phenyl-2-thiourea (Sigma-Aldrich) to prevent melanization and incubated for 4 days at 28°C. After the incubation period, infected and uninfected morphants, mutants and their controls were imaged and groups of 30 embryos were snap-frozen in liquid nitrogen and RNA was isolated for Illumina RNAseq analysis.

Repositories


GEO

GSE58230

ENA

SRP042975

BioProject

PRJNA251728

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Contact


Annemarie Meijer