Computational protocol: Structure–Function Analysis of the Non-Muscle Myosin Light Chain Kinase (nmMLCK) Isoform by NMR Spectroscopy and Molecular Modeling: Influence of MYLK Variants

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Protocol publication

[…] Protein homology modeling was performed using Swiss-Model []. Specifically, we identified the structures as optimal templates that span the region of interest within the N-terminus nmMLCK1 as well as possess the highest sequence identity, with the quality of model assessable by QMEAN Z-score []. The results were processed and visualized using PyMol (The PyMOL Molecular Graphics System, Version 1.5.0.4 Schrödinger, LLC.). The root-mean-square deviation (RMSD) values were obtained using SuperPose []. […]

Pipeline specifications

Software tools SWISS-MODEL, QMEAN, PyMOL, SuperPose
Application Protein structure analysis
Diseases Asthma, Pneumonia, Acute Lung Injury
Chemicals Amino Acids