Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 12
Release date: Jan 1 2014
Last update date: Apr 2 2014
Access: Public
Chemicals: Dexamethasone
Dataset link Expression profile pAP1-AP1-GR ap1cal inflorescences - time series (days)

Experimental Protocol


Four sets of biologically independent tissue samples were collect before DEX-induction (day 0, 0d), as well as at 2 days, 4 days and 8 days after the treatment. Samples for each timepoint and biological replicate were co-hybridized as indicated; one sample was labeled with Cy3 and the other with Cy5. The dyes used for labeling RNA from a given timepoint were switched for two of the replicate experiments, to reduce dye-related artifacts. This experimental setup resulted in a total of 3 hybridizations per set (0days vs 2days, 2days vs 4days, and 4days vs 8days), and an even number (2) of hybridizations of each dye polarity per comparison.

Repositories


GEO

GSE47981

ArrayExpress

E-GEOD-47981

BioProject

PRJNA208520

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Contact


Jose Luis Riechmann