|Number of samples:||2|
|Release date:||Jun 20 2013|
|Last update date:||Sep 22 2013|
|Dataset link||Global transcriptome analysis of orange wheat blossom midge, Sitodiplosis mosellana (Gehin) (Diptera: Cecidomyiidae) to identify candidate transcripts regulating diapause|
Examination of different gene expression in DOL and NDOL Total RNA was extracted separately from DOL and NDOL using Trizol Reagent following manufacturer’s instructions. Approximately 10μg RNA from each sample was used to construct individual DGE libraries. mRNA was isolated as described under cDNA library construction. The cDNA fragments were purified by agarose gel electrophoresis and enriched by PCR amplification. Each library had an insert size of 200 bp, and 42 - 50 bp sequences were generated by Illumina HiSeq 2000 in a single run using paired-end technology. SRA Study SRP019201.