Computational protocol: A Loop-Mediated Isothermal Amplification Assay for Rapid Detection of Cyprinid Herpesvirus 2 in Gibel Carp (Carassius auratus gibelio)

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Protocol publication

[…] A PCR primer set (JF/JR) was designed to amplify the complete cds of the viral putative DNA helicase gene (GenBank accession EU349287). Then nested PCR primers (JF1/JR1, JF2/JR2) were designed for detection (GenBank accession KC245087) by software Primer Premier 5.0.A set of four LAMP primers (JF3 and JB3, JBIP and JFIP) recognizing six distinct regions in the viral putative DNA helicase gene sequence (GenBank accession KC245087) was designed by using the Primer Explorer version 4 (http://primerexplorer.jp/elamp4.0.0/index.html). The primers' sequences and the locations were indicated in . All primers were synthesized (Invitrogen, Shanghai, China) and are shown in . […]

Pipeline specifications

Software tools Primer Premier, PrimerExplorer
Application qPCR
Organisms Cyprinid herpesvirus 2, Carassius gibelio, Cyprinid herpesvirus 3
Chemicals Magnesium Chloride