Dataset features

Specifications


Application: RNA-seq analysis
Number of samples: 15
Release date: Sep 4 2015
Last update date: Oct 24 2018
Access: Public
Diseases: Barrett Esophagus, Corneal Diseases
Dataset link Transcriptomic analysis of cultured corneal endothelial cells as a validation for their use in cell-replacement therapy

Experimental Protocol


Transcriptomes from ex vivo corneal endothelium, primary cultures and three cell lines were compared. Three samples of each endothelial cell group were submitted for RNA sequencing for a total of 15 samples. The transcriptome for the ex vivo corneal endothelium was used as the reference (i.e., proxy for in vivo corneal endothelium). Transcript abundances for a subset of genes associated with corneal endothelial cell function or disease were validated with qPCR and western blot. Samples of ex vivo endothelium used for validation were independent replicates not used for RNA-sequencing.

Repositories


GEO

GSE65991

ArrayExpress

E-GEOD-65991

ENA

SRP055101

BioProject

PRJNA275652

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Contact


Anthony Aldave
Anthony J. Aldave