Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 24
Release date: Mar 21 2018
Last update date: Oct 29 2018
Access: Public
Chemicals: Amino Acids, Carbon, Nucleotides, Oxygen
Dataset link Gene expression analysis upon mtDNA depletion [microarray]

Experimental Protocol


We analyzed HEK293 cells in which mtDNA was depleted by doxycycline addition to the medium to turn a dominant negative mitochondrial DNA polymerase (DN-POLG). Samples were analyzed at days 0, 3, 6 and 9 for a total of 4 samples. Each time point was done in three indepedent biological replicates. Time point 0 was used as controls with samples being un-induced. To determine the TCA cycles effects, HEK293 DN-POLG overexpressing AOX/NDI1 were also included at days 0 and 9, with 3 biological replicates to each Gene expression was also evaluated in 143B cells in which mtDNA was chronically depleted based on ethidium bromide treatment. Samples include 3 independent biological replicates of controls (rho+) and 3 of mtDNA depleted (rho0).

Repositories


GEO

GSE99834

BioProject

PRJNA389740

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Contact


Janine Santos

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