Dataset features

Specifications


Application: ChIP-on-chip analysis
Number of samples: 12
Release date: Apr 25 2014
Last update date: Apr 26 2014
Access: Public
Diseases: Breast Neoplasms
Chemicals: Etoposide
Dataset link BCLAF1 Chip-chip control (siGFP) and BRCA1 depleted (siBRCA1) 293T cells in the absense or presense of Etoposide

Experimental Protocol


Chromatin Immunoprecipitaitons were performed in triplicate with BCLAF1 antibodies in control 293T cells transfected with siGFP siRNAs and BRCA1 siRNAs (siBRCA1 to deplete BRCA1). Immunoprecipitated genomic DNA was labelled with Cy3 and Input genomic DNA was labelled with Cy5 and hybridized to NimbleGen human 3x720k RefSeq promoter arrays to identify BCLAF1 boundgenomic DNA regions.

Repositories


GEO

GSE47016

ArrayExpress

E-GEOD-47016

BioProject

PRJNA203287

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Contact


Kienan Savage