Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 18
Release date: Jan 1 2010
Last update date: Mar 21 2012
Access: Public
Genes: KAT2A
Taxon: Saccharomyces cerevisiae, Schizosaccharomyces pombe
Dataset link Comparison of gene regulation by Gcn5 under KCl stress in S. cerevisiae and S. pombe

Experimental Protocol


Gene expression profiling experiment in which gene expression in logarithmically growing cultures of test groups was compared to that of the control groups in rich medium at 25C. There are 6 subsets in total, 3 subsets in each yeast including the following: 1 gcn5 mutant compared to wt under rich medium without treatment, 2 gcn5 mutant compared to wild type both under KCl treatment, 3 wild type strains under KCl treatment compared to wild type without treatment. RNA was extracted and subjected to cDNA expression profiling analysis using the established protocols (Xue et al, 2004). Independent RNA preparations were used to hybridize to microarray slides with dye swap. Data normalized with 'Lowess' per chip per spot normalization.

Repositories


GEO

GSE5218

ArrayExpress

E-GEOD-5218

BioProject

PRJNA122641

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Contact


Anthony Wright

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