Dataset features

Specifications


Application: RNA-seq analysis, sRNA-seq analysis
Number of samples: 2
Release date: Jul 28 2014
Last update date: Mar 3 2015
Access: Public
Taxon: Apis mellifera
Dataset link Small RNA expression and caste differentiation in the honeybee

Experimental Protocol


Three healthy 10-frame colonies of ‘Zhenongda No.1’- a high-yielding royal jelly breed of Apis mellifera ligustica , were maintained at the Huajiachi campus of Zhejiang University. In each colony, the queen laid eggs over a period of 24 hours in one empty frame which was subsequently moved to an egg-free super-chamber. After 66 hours (less than 18 hours after hatching), we transferred 150 larvae into queen cups to rear queens in each colony and put the queen cup frames into their corresponding colonies. 40-60 worker larvae and queen larvae were collected from each colony after 4 days (73~90 h after hatching), 5 days (97~114 h after hatching) and 6 days (121~138 h after hatching). The larval samples were collected into 50 ml tubes, immediately frozen in liquid nitrogen and stored at -80C until being used for RNA extraction. After total RNA was extracted  and quantified , relative equal amounts of total RNAs from each of the three sampling days were pooled into respectively worker and queen samples, and the fraction of small RNAs less than 30nt long was retained and sequenced on the Illumina/Solexa high-throughput platform (HTP).

Repositories


GEO

GSE52630

ArrayExpress

E-GEOD-52630

ENA

SRP033246

BioProject

PRJNA229500

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Contact


Xiangqian Guo

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