Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 24
Release date: Jan 10 2018
Last update date: Mar 23 2018
Access: Public
Diseases: Astrocytoma, Sarcoma, Tumor Lysis Syndrome
Chemicals: Doxycycline
Dataset link Dysregulated Signalling leads to altered cell migration: an oncogenic basis for the development of CCSK

Experimental Protocol


Gene expression changes resulting from induction of the fusion gene were compared in mock- and doxycycline treated HEK293 and BJ-hTERT HA-YWHAE-NUTM2 cell lines at 24 and 72 hours. Total RNA was extracted from cell lines, assayed by NanoDrop (Thermo Scientific) and 200 ng was used for gene expression analysis using the Affymetrix® Human Gene 2.1 ST Array (Affymetrix, Santa Clara, CA, USA). The arrays were performed in triplicate and analysed using Bioconductor packages ‘oligo’ and ‘limma”.

Repositories


GEO

GSE98757

BioProject

PRJNA386119

Download


Contact


Maureen O'Sullivan