Dataset features

Specifications


Dataset type: Expression profiling by high throughput sequencing
Number of samples: 5
Release date: Feb 7 2019
Last update date: Feb 18 2019
Access: Public
Dataset link Epigenetic signatures associated with paternally-expressed imprinted genes in the endosperm

Experimental Protocol


Col × Ler reciprocal crosses were performed using Arabidopsis lines expressing PHE1::NTF and PHE1::BirA. 4DAP siliques were collected and tissue homogenization and nuclei purification were performed from three biological replicates for LerxCol and two for ColxLer using INTACT. Total RNA was extracted from purified nuclei using the mirVana Isolation Kit Protocol (Ambion). mRNA extraction was performed using NEBNext Poly(A) mRNA Magnetic Isolation and the Libraries were prepared with the NEBNext Ultra II RNA Library Prep Kit from Illumina. Samples were sequenced at the National Genomic Infrastructure (NGI) from SciLife Laboratory (Uppsala, Sweden) on an Illumina HiSeq2500 in paired-end 125bp read length.

Repositories


GEO

GSE119915

ENA

SRP161686

BioProject

PRJNA490691

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Contact


Juan Santos-González