Dataset features

Specifications


Application: ChIP-seq analysis
Number of samples: 4
Release date: May 11 2013
Last update date: Nov 3 2017
Access: Public
Taxon: Caenorhabditis elegans
Dataset link Snyder_NHR-76_GFP_L4

Experimental Protocol


EXPERIMENT TYPE: CHIP-seq. BIOLOGICAL SOURCE: Strain: OP203(official name : OP203 genotype : unc-119(ed3); wgIs203(nhr-76::TY1 EGFP FLAG; unc-119) outcross : 3 mutagen : Bombard tags : GFP::3xFlag description : This strain's transgene was constructed by Mihail Sarov at the Max Planck Institute for Cell Biology in Tubiginen using Tony Hyman's recombineering pipeline. The resulting plasmid was used for biolistic transformation of an unc-119(ed3) strain. The NHR-76::EGFP fusion protein is expressed in the correct nhr-76 spatio-temporal expression pattern. This strain was used for ChIP-seq experiments to map the in vivo binding sites for the NHR-76 transcription factor. made_by : R. Waterston ); Developmental Stage: L4; Genotype: unc-119(ed3); wgIs203(nhr-76::TY1 EGFP FLAG; unc-119); Sex: Hermaphrodite; EXPERIMENTAL FACTORS: Developmental Stage L4; Target gene nhr-76; Strain OP203(official name : OP203 genotype : unc-119(ed3); wgIs203(nhr-76::TY1 EGFP FLAG; unc-119) outcross : 3 mutagen : Bombard tags : GFP::3xFlag description : This strain's transgene was constructed by Mihail Sarov at the Max Planck Institute for Cell Biology in Tubiginen using Tony Hyman's recombineering pipeline. The resulting plasmid was used for biolistic transformation of an unc-119(ed3) strain. The NHR-76::EGFP fusion protein is expressed in the correct nhr-76 spatio-temporal expression pattern. This strain was used for ChIP-seq experiments to map the in vivo binding sites for the NHR-76 transcription factor. made_by : R. Waterston ); temp (temperature) 20 degree celsius

Repositories


GEO

GSE46769

ArrayExpress

E-GEOD-46769

ENA

SRP022264

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DCC modENCODE