Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 4
Release date: Nov 30 2012
Last update date: Nov 30 2012
Access: Public
Diseases: Tularemia
Dataset link Francisella Expression Microarray Analysis

Experimental Protocol


A whole genome NibleGen microarray was developed for multiple Francisella subspecies, using shared probes for common genes as well as unique probes for subspecies-specific genes. Included were probes representing the F. novicida U112 genome, with a maximum of 6 probes/gene. RNA was isolated from bacteria and purified as described above. The double-strand cDNA was generated and labeled with Cy3 according to the NimbleGen Gene Expression Array protocol (www.nimblegen.com), using SuperScript Double-Stranded cDNA Synthesis Kit (Invitrogen) and the DNA Labeling Kit (NimbleGen). Microarray results were analyzed using the ArrayStar software (DNASTAR).

Repositories


GEO

GSE39647

ArrayExpress

E-GEOD-39647

BioProject

PRJNA171329

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Contact


Xhavit Zogaj