|Number of samples:||14|
|Release date:||Aug 26 2018|
|Last update date:||Nov 30 2018|
|Dataset link||The RNA-binding protein ROD1/PTBP3 cotranscriptionally defines AID-loading sites to mediate antibody class switch in mammalian genomes|
ROD1/PTBP3 CLIP-seq was performed with naïve splenic B cells and LPS-activated B cells, and two biological replicates were labeled as Rep1 and Rep2. Two biological repeats of AID CLIP-seq in LPS-activated B cells were labeled as Rep1 and Rep2; in addition, two AID CLIP-seq libraries were also generated upon the depletion of ROD1/PTBP3. For comparison, IgG antibodies were applied to generate two replicates of CLIP-seq libraries for negative control purposes. To examine whether ROD1/PTBP3 ablation alter the global nascent RNA levels, two biological repeats of GRO-seq were performed on LPS-activated B cells.