Computational protocol: RecOR complex including RecR N-N dimer and RecO monomer displays a high affinity for ssDNA

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Protocol publication

[…] The structure of full-length TTERecR was solved by the molecular replacement method using PHASER () with one monomer of drRecR [Protein Data Bank (PDB):1VDD] as the search model at 20-3 Å resolution. The model was completed and refined using the REFMAC5 refinement (,) and COOT () at 20-2.45 Å resolution. The structures of the mutants TTERecR16-196 and TTERecRK21G were solved using the model of TTERecR, and refined using the REFMAC5 or PHENIX refinement programs (). All structural images were drawn using PyMOL (http://www.pymol.org/). Detailed crystallographic statistics are shown in . Coordinates have been deposited into PDB under the accession codes: 3VDP, 3VE5 and 3VDU. […]

Pipeline specifications

Software tools REFMAC5, Coot, PHENIX, PyMOL
Application Protein structure analysis
Organisms Caldanaerobacter subterraneus subsp. tengcongensis