Computational protocol: Study on the kinetics and influence of feline platelet aggregation and deaggregation

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Protocol publication

[…] Statistical analyses were performed with the software packages R and nlme []. Linear mixed-effects models were performed to model the outcome variables PLT and WBC counts measured by the Sysmex XT-2000iV and the Mythic 18 instrument with the potential explanatory variables’ presence (and magnitude/importance) of aggregates (classified into the three groups of “no aggregates”, “low to moderate number of aggregates” and “high/important number of aggregates”), anaesthesia (yes or no), quality of blood collection (classified into “without any problem” and “difficult”) and the nine different time points. Model selection and validation was based on Akaike’s information criterion AIC and by checking visually the residuals for normality, homogeneity and independence. Significant differences were set at p < 0.05. For both instruments, the final models contained as explanatory variables the presence of aggregates, time, bleeding, anaesthesia, interaction between presence of aggregates and time, interaction between the presence of aggregates and bleeding as fixed effects and the individual cats as random effects. The random structure was extended using different variances for the groups of aggregates for the Sysmex XT-2000iV and different variances for the time points for the Mythic 18. For the time-correlated measurements, an AR-1 correlation structure was added. Linear mixed effects models were performed to model the outcome variable glucose by the potential explanatory variables of aggregates, time, anaesthesia and quality of blood collection. Glucose concentration under the detection limit (<0.6 mmol/l) of the instrument was set arbitrarily at 0.5 mmol/l. Bar charts were made using GraphPad Prism (version 3.00 for Windows, GraphPad Software). […]

Pipeline specifications

Software tools nlme, lme4
Application Mathematical modeling
Organisms Felis catus
Diseases Blood Platelet Disorders, Thrombocytopenia
Chemicals Edetic Acid, Glucose