Computational protocol: Long Term In Vitro Expansion of Salivary Gland Stem Cells Driven by Wnt Signals

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Protocol publication

[…] Immunofluorescence images from tissue sections and images of cultivated cells were acquired with a Leica TCS Sp8 confocal microscope. Images of time-lapse experiments were acquired with an Olympus IMT-2 inverted microscope. Immunohistochemical images of tissue sections were acquired with a Leica 6000 series microscope or a Tissuegnostics TissueFAXS high-throughput fluorescence microscope. Co-localization of two proteins was quantified by the ImageJ “Colocalization_Finder” plugin (Christophe Laummonerie 2006/08/29: Version 1.2). Immunofluorescence images were reconstructed and analyzed using Imaris (Bitplane) software. […]

Pipeline specifications

Software tools ImageJ, Imaris
Applications Laser scanning microscopy, Microscopic phenotype analysis
Organisms Mus musculus