|Application:||Gene expression microarray analysis|
|Number of samples:||27|
|Release date:||Jun 7 2012|
|Last update date:||Oct 29 2018|
|Diseases:||Anemia, Sickle Cell, Hypertension, Pulmonary|
|Dataset link||A Validated Gene Signature from Peripheral Blood Mononuclear Cells in Pulmonary Hypertension Associated with Sickle Cell Disease|
Total RNA was isolated from PBMCs using standard molecular biology protocols without DNA contamination or RNA degradation. Sample processing (e.g., cDNA generation, fragmentation, end labeling, hybridization to Affymetrix GeneChip Human Exon 1.0 ST arrays) was performed per manufacturer’s instructions. A total of 27 African descent American patients with sickle cell disease were included in the microarray analysis.