Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 27
Release date: Jun 7 2012
Last update date: Oct 29 2018
Access: Public
Diseases: Anemia, Sickle Cell, Hypertension, Pulmonary
Dataset link A Validated Gene Signature from Peripheral Blood Mononuclear Cells in Pulmonary Hypertension Associated with Sickle Cell Disease

Experimental Protocol


Total RNA was isolated from PBMCs using standard molecular biology protocols without DNA contamination or RNA degradation. Sample processing (e.g., cDNA generation, fragmentation, end labeling, hybridization to Affymetrix GeneChip Human Exon 1.0 ST arrays) was performed per manufacturer’s instructions. A total of 27 African descent American patients with sickle cell disease were included in the microarray analysis.

Repositories


GEO

GSE38528

ArrayExpress

E-GEOD-38528

BioProject

PRJNA168166

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Contact


Tong Zhou