Dataset features

Specifications


Application: Gene expression microarray analysis, ChIP-on-chip analysis
Number of samples: 4
Release date: Apr 11 2009
Last update date: Mar 21 2012
Access: Public
Dataset link An Rtt109-independent role for Vps75 in transcription-associated nucleosome dynamics

Experimental Protocol


ChIP-chip analysis of the distribution of Vps75 on budding yeast chromosomes. Experiment type: ChIP-chip = Chromatin immunoprecipitation (ChIP) followed by hybridization to a high-density oligonucleotide microarray (chip). Number of hybridizations performed: 2 ChIP-chip samples and 2 SUPernatant samples for each type of tiling array (whole genome and chromosome VI). Analysis: ChIP samples were normalized against SUPernatant fractions. Quality control: Confirmation of results by duplication of experiments and by hybridization to two different types of Saccharomyces cerevisiae tiling arrays (whole genome and chromosome VI). Also, whole cell extract, SUPernatant, and ChIP fractions were checked by Western blotting.

Repositories


GEO

GSE15607

BioProject

PRJNA116685

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Contact


Luke Selth