|Application:||Gene expression microarray analysis|
|Number of samples:||27|
|Release date:||Nov 1 2009|
|Last update date:||Jul 31 2017|
|Diseases:||Hyper-IgM Immunodeficiency Syndrome|
|Dataset link||Expression data from adult rat tail MNs after spinal cord transection|
Tail MNs were retorgradely labelled with flourogold injected into the muscle and intra peritoneally. 5-7 days after tracer injections the spinal cord was dissected out, snab frozen in liquid nitrogen, sliced in 10 um thick slices and fluorescent motor neurons were laser dissected into a collector tube to a total of ca. 50-200 cells pr sample. RNA was then extracted, two round amplified and hybridized to Affymetrix rat 230 2.0 arays. 27 samples were hybridized onto chips, 8 Spi-21, 8 Spi-60, 6 ShamC-21 and 5 ShamC-60.