Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 27
Release date: Nov 1 2009
Last update date: Jul 31 2017
Access: Public
Diseases: Hyper-IgM Immunodeficiency Syndrome
Dataset link Expression data from adult rat tail MNs after spinal cord transection

Experimental Protocol


Tail MNs were retorgradely labelled with flourogold injected into the muscle and intra peritoneally. 5-7 days after tracer injections the spinal cord was dissected out, snab frozen in liquid nitrogen, sliced in 10 um thick slices and fluorescent motor neurons were laser dissected into a collector tube to a total of ca. 50-200 cells pr sample. RNA was then extracted, two round amplified and hybridized to Affymetrix rat 230 2.0 arays. 27 samples were hybridized onto chips, 8 Spi-21, 8 Spi-60, 6 ShamC-21 and 5 ShamC-60.

Repositories


GEO

GSE16710

ArrayExpress

E-GEOD-16710

BioProject

PRJNA117361

Download


Contact


Jesper Ryge