Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 4
Release date: Mar 16 2009
Last update date: Jan 18 2013
Access: Public
Diseases: Brain Edema, Brain Ischemia
Dataset link Effect of Rac1 haploinsufficiency on the gene expression profile of mouse endothelial cells

Experimental Protocol


Rac1+/+ and Rac1+/- ECs were cultured to subconfluence. Total RNA was extracted and reverse transcribed. Rac1+/+ (n = 2) and Rac1+/- (n = 2) EC cDNAs were labeled with Cy3 and Cy5, respectively, and equimolar mixtures of labeled Rac1+/+ and Rac1+/- probes were hybridized to 2 slides (sample #21648, #21649). Additionally, Rac1+/+ (n = 2) and Rac1+/- (n=2) EC cDNAs labeled with Cy5 and Cy3 (dye-swapping) were hybridized to 2 slides (sample #21650, #21651). Comparison of signal intensities between Rac1+/+ (channel 2) and Rac1+/- (channel 1) was conducted on each slide (sample), and the ratio of intensities from 4 slides were statistically assessed for each gene feature to investigate differential gene expression.

Repositories


GEO

GSE15003

ArrayExpress

E-GEOD-15003

BioProject

PRJNA114719

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