Computational protocol: A wild-type Botrytis cinerea strain co-infected by double-stranded RNA mycoviruses presents hypovirulence-associated traits

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Protocol publication

[…] The cDNA synthesis, cloning and sequencing was performed as described previously by Potgieter et al., []. The sequences of capsid protein of twenty-five partitiviruses, plus the sequence of the L-A totivirus [] and the sequence of Bc378V1, were aligned using the software Clustal X []. To obtain phylogenetic relationships, alignments were analyzed by the neighbor joining method, using the MEGA 5.0 program []. The robustness of the branches generated by the analysis, were determined with 1000 bootstrap replicates. […]

Pipeline specifications

Software tools Clustal W, MEGA
Application Phylogenetics
Organisms Botrytis cinerea