Dataset features

Specifications


Application: RNA-seq analysis
Number of samples: 6
Release date: Nov 12 2017
Last update date: Jul 26 2018
Access: Public
Chemicals: Arsenic
Computational protocol: FastQC, Trimmomatic, TopHat, HTSeq, DESeq2, GEO
Dataset link Measuring differential gene expression with RNA-seq in 1mM inorganic arsenic exposed and conreol sibling Tg(fabp10:nls-mcherry) livers at 5dpf

Experimental Protocol


10-20 livers from 5dpf embryos were pooled per sample of either control or 1 mM inorganic arsenic exposed Tg(fabp10:nls-mcherry) zebrafish larvae and RNA was extracted using the Zymo Quick-RNA Micro Kit with on-column DNase treatment. Libraries were prepared according to Illumina Truseq RNA sample prep kit, version 2, followed by Ribo-Zero Gold treatment.

Repositories


GEO

GSE104953

ENA

SRP119921

BioProject

PRJNA414225

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