Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 8
Release date: Jun 15 2016
Last update date: Feb 2 2018
Access: Public
Diseases: Adenoviridae Infections, Neoplasms, Tumor Lysis Syndrome
Dataset link Development of gene expression profiles for murine breast tumours overexpression the transcription factor PRH

Experimental Protocol


Total RNA was extracted from each tumour using the Qiagen miRNAeasy kit, processed into cRNA using the Agilient low input, one colour labelling kit, hybridised onto the 8 x 66k whole human genome expression microarrays. Data was normalised in R64 and comparison matrices between matched Tumour and Healthy endothelum were constructed. Genes upregualated in all the comparisons were taken forward for further analysis.

Repositories


GEO

GSE83319

ArrayExpress

E-GEOD-83319

BioProject

PRJNA325586

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Contact


Joseph Wragg
Joseph William Wragg