|Application:||Gene expression microarray analysis|
|Number of samples:||12|
|Release date:||Apr 4 2007|
|Last update date:||Mar 16 2012|
|Diseases:||Neoplasms, Tumor Lysis Syndrome, Carcinoma, Lewis Lung|
|Dataset link||Profiling of genes regulated by TGF-beta in lung carcinoma (A549) and immortalized lung epithelial (HPL1D)cells.|
To compare the genes regulated by TGF-beta in non transformed and transformed cells, we chose an immortalized lung epithelial cell line, HPL1D and a lung adenocarcinoma cell line, A549. Cells were plated and propagated in designated media and TGF-beta treatments were carried out in serum free conditions at various time intervals, 1hr, 4hr and 12hr. The RNA was extracted and expression profiling was performed using microarray experiments on 19K cDNA arrays after labeling the cDNAs with Cy3 and Cy5 dyes for normal and tumor cell RNAs respectively. The image and data analysis was performed using Quantarray (P.E. Life Sciences, USA) and Avadis (Strand Life Sci. India) softwares. The results are computed as log2 transformed fold changes between treated and untreated respective cell lines. The data of regulated genes of each cell line was compared with the other cell line and exclusively and commonly regulated genes have been determined.
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