Computational protocol: Expression of surfactant protein B is dependent on cell density in H441 lung epithelial cells

Similar protocols

Protocol publication

[…] Immunoblotting was performed as previously described []. Blots were probed with primary antibodies against SP-B, claudin-5, claudin-8, and β-actin overnight at 4°C, followed by staining with corresponding IRDye® secondary antibodies (LI-COR Inc.) or an horseradish peroxidase conjugated goat anti rabbit IgG (Thermo Fisher Scientific) for 1 h at room temperature. Specific protein bands were visualized using an ODYSSEY® Infrared Imaging System (LI-COR Inc.) or a ChemiDoc™ MP Imaging System (Bio-Rad Laboratories, Hercules, CA). Captured signals were quantified by densitometric analysis using Image Studio Lite v5.0.21 (LI-COR Inc.) or Image Lab™ Software v5.2.1 (Bio-Rad Laboratories). […]

Pipeline specifications

Software tools Image Studio, Image Lab
Application DNA fingerprinting
Chemicals Dactinomycin, Dexamethasone