Computational protocol: Chromosomal Organization and Segregation in Pseudomonas aeruginosa

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Protocol publication

[…] Strains were grown overnight in LB, diluted 300 times in minimal medium A supplemented with either 0.25% citrate or 0.12% casamino acids and 0.5% glucose (when looking at chromosomal tags, 0.5 mM IPTG was added to growth medium) until they reach an OD600 comprised between 0.05 and 0.1. Cells were then spread on agarose pads and immediately observed using a Leica DM6000 microscope, a coolsnap HQ CCD camera (Roper) and Metamorph software. Image analysis was performed using the MATLAB-based software MicrobeTracker Suite . […]

Pipeline specifications

Software tools MetaMorph, MicrobeTracker
Application Conventional fluorescence microscopy
Organisms Pseudomonas aeruginosa