Computational protocol: New Insights into the Hendra Virus Attachment and Entry Process from Structures of the Virus G Glycoprotein and Its Complex with Ephrin-B2

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Protocol publication

[…] Crystals were cryo-protected in mother-liquor with 25% glycerol added. X-ray diffraction data were collected at the Northeastern Collaborative Access Team, Advance Photon Source beamline 24ID-C. The diffraction images were integrated and scaled with DENZO/SCALEPACK . The phases were determined by molecular replacement using Phaser with NiV-G (3D11) and the NiV-G/ephrin-B3 complex (3D12) as search models. The program package PHENIX was used for structure refinement. Manual model building was carried out with the program O . Figures were generated using PyMOL. […]

Pipeline specifications

Software tools PHENIX, PyMOL
Applications Small-angle scattering, Protein structure analysis
Organisms Hendra henipavirus, Human poliovirus 1 Mahoney
Diseases HIV Infections