Computational protocol: Binding of NUFIP2 to Roquin promotes recognition and regulation of ICOS mRNA

Similar protocols

Protocol publication

[…] All mRNA and sequence data used in this study were acquired from the NCBI Reference Sequence Database (RefSeq) collection release 69. A data set was prepared by extracting all mRNAs of ICOS. Only mRNAs with a completely annotated 3′-UTR were used. The data set included 24 mammals. A complete list of species and associated identification numbers used in this study is given as Supplementary Table .A combined multiple sequence-structure alignment of the data set sequences were obtained applying LocARNA version 1.8.1 with the option local-progressive followed by RNAalifold from the ViennaRNA package version 2.1.8-2. […]

Pipeline specifications

Software tools LocARNA, RNAalifold, ViennaRNA
Application RNA structure analysis