Computational protocol: Vertical Transmission of H9N2 Avian Influenza Virus in Goose

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Protocol publication

[…] Nucleotide sequence analysis was performed based on the complete HA gene of H9N2 AIV. Sequences from the newly isolated strains (from vitelline membrane, embryo, allantoic fluid, and goslings) were compared to the original DP01 isolate. The alignment was constructed using the ClustalW method of MegAlign program of DNA Star software (Doan et al., ). Phylogenetic comparisons were conducted using neighbor-joining analysis by MEGA 5.05 software (Tamura et al., ), where bootstrap confidence values were determined using 1,000 replicates. […]

Pipeline specifications

Software tools Clustal W, MEGA
Application Phylogenetics
Diseases Infection, HIV Infections
Chemicals Phosphates